@Article{JFBI-5-2,
author = {},
title = {Chemical Modifications of Electrospun Non-woven Hydroxypropyl Cellulose Fabrics for Immobilization of Aminoacylase-I},
journal = {Journal of Fiber Bioengineering and Informatics},
year = {2012},
volume = {5},
number = {2},
pages = {191--205},
abstract = {Because of its wide solubility range, Hydroxypropyl Cellulose (HPC) is suitable for fabricating fine-fiber
materials via electrospinning. The resulting electrospun non-woven fabric (HPC-ESNW) requires an
appropriate post-spinning treatment for applications in aqueous environments. In the present study, we
examined the insolubilization of HPC-ESNW via cross-linking using bifunctional isocyanates.
Modification of the fine-fiber surfaces with NCO groups enables introduction of cationic functionalities;
we found that cationic Diethylaminoethyl (DEAE) groups are suitable for immobilization of aminoacylase-
I onto these fine-fiber surfaces under mild conditions. The NCO groups can be also converted to amino
groups, followed by activation with bifunctional N-hydroxysuccinimide (NHS) esters. The NHS-ESNW
can chemically bind the aminoacylase-I. Two kinds of immobilized enzyme were tested for stereospecific
recognition of a substrate and for immobilized activity yields. The results suggest that NCO-ESNW is
a multi-purpose intermediate for chemical modification of ESNW and that the hydrophilic HPC-ESNW
is a promising material for use as a matrix for enzyme immobilization.},
issn = {2617-8699},
doi = {https://doi.org/10.3993/jfbi06201208},
url = {https://global-sci.com/article/86751/chemical-modifications-of-electrospun-non-woven-hydroxypropyl-cellulose-fabrics-for-immobilization-of-aminoacylase-i}
}