Volume 5, Issue 2
Chemical Modifications of Electrospun Non-woven Hydroxypropyl Cellulose Fabrics for Immobilization of Aminoacylase-I

Viswanathamurthi Periasamy, Kesavan Devarayan, Masakazu Hachisu, Jun Araki & Kousaku Ohkawa

Journal of Fiber Bioengineering & Informatics, 5 (2012), pp. 191-205.

Published online: 2012-05

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  • Abstract
Because of its wide solubility range, Hydroxypropyl Cellulose (HPC) is suitable for fabricating fine-fiber materials via electrospinning. The resulting electrospun non-woven fabric (HPC-ESNW) requires an appropriate post-spinning treatment for applications in aqueous environments. In the present study, we examined the insolubilization of HPC-ESNW via cross-linking using bifunctional isocyanates. Modification of the fine-fiber surfaces with NCO groups enables introduction of cationic functionalities; we found that cationic Diethylaminoethyl (DEAE) groups are suitable for immobilization of aminoacylase- I onto these fine-fiber surfaces under mild conditions. The NCO groups can be also converted to amino groups, followed by activation with bifunctional N-hydroxysuccinimide (NHS) esters. The NHS-ESNW can chemically bind the aminoacylase-I. Two kinds of immobilized enzyme were tested for stereospecific recognition of a substrate and for immobilized activity yields. The results suggest that NCO-ESNW is a multi-purpose intermediate for chemical modification of ESNW and that the hydrophilic HPC-ESNW is a promising material for use as a matrix for enzyme immobilization.
  • Keywords

Aminoacylase Chemical modification Electrospinning Hydroxypropyl cellulose Immobilization

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@Article{JFBI-5-191, author = {}, title = {Chemical Modifications of Electrospun Non-woven Hydroxypropyl Cellulose Fabrics for Immobilization of Aminoacylase-I}, journal = {Journal of Fiber Bioengineering and Informatics}, year = {2012}, volume = {5}, number = {2}, pages = {191--205}, abstract = {Because of its wide solubility range, Hydroxypropyl Cellulose (HPC) is suitable for fabricating fine-fiber materials via electrospinning. The resulting electrospun non-woven fabric (HPC-ESNW) requires an appropriate post-spinning treatment for applications in aqueous environments. In the present study, we examined the insolubilization of HPC-ESNW via cross-linking using bifunctional isocyanates. Modification of the fine-fiber surfaces with NCO groups enables introduction of cationic functionalities; we found that cationic Diethylaminoethyl (DEAE) groups are suitable for immobilization of aminoacylase- I onto these fine-fiber surfaces under mild conditions. The NCO groups can be also converted to amino groups, followed by activation with bifunctional N-hydroxysuccinimide (NHS) esters. The NHS-ESNW can chemically bind the aminoacylase-I. Two kinds of immobilized enzyme were tested for stereospecific recognition of a substrate and for immobilized activity yields. The results suggest that NCO-ESNW is a multi-purpose intermediate for chemical modification of ESNW and that the hydrophilic HPC-ESNW is a promising material for use as a matrix for enzyme immobilization.}, issn = {2617-8699}, doi = {https://doi.org/10.3993/jfbi06201208}, url = {http://global-sci.org/intro/article_detail/jfbi/4874.html} }
TY - JOUR T1 - Chemical Modifications of Electrospun Non-woven Hydroxypropyl Cellulose Fabrics for Immobilization of Aminoacylase-I JO - Journal of Fiber Bioengineering and Informatics VL - 2 SP - 191 EP - 205 PY - 2012 DA - 2012/05 SN - 5 DO - http://doi.org/10.3993/jfbi06201208 UR - https://global-sci.org/intro/article_detail/jfbi/4874.html KW - Aminoacylase KW - Chemical modification KW - Electrospinning KW - Hydroxypropyl cellulose KW - Immobilization AB - Because of its wide solubility range, Hydroxypropyl Cellulose (HPC) is suitable for fabricating fine-fiber materials via electrospinning. The resulting electrospun non-woven fabric (HPC-ESNW) requires an appropriate post-spinning treatment for applications in aqueous environments. In the present study, we examined the insolubilization of HPC-ESNW via cross-linking using bifunctional isocyanates. Modification of the fine-fiber surfaces with NCO groups enables introduction of cationic functionalities; we found that cationic Diethylaminoethyl (DEAE) groups are suitable for immobilization of aminoacylase- I onto these fine-fiber surfaces under mild conditions. The NCO groups can be also converted to amino groups, followed by activation with bifunctional N-hydroxysuccinimide (NHS) esters. The NHS-ESNW can chemically bind the aminoacylase-I. Two kinds of immobilized enzyme were tested for stereospecific recognition of a substrate and for immobilized activity yields. The results suggest that NCO-ESNW is a multi-purpose intermediate for chemical modification of ESNW and that the hydrophilic HPC-ESNW is a promising material for use as a matrix for enzyme immobilization.
Viswanathamurthi Periasamy, Kesavan Devarayan, Masakazu Hachisu, Jun Araki & Kousaku Ohkawa. (2019). Chemical Modifications of Electrospun Non-woven Hydroxypropyl Cellulose Fabrics for Immobilization of Aminoacylase-I. Journal of Fiber Bioengineering and Informatics. 5 (2). 191-205. doi:10.3993/jfbi06201208
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